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. 2019 Sep 5;2019:8030697. doi: 10.1155/2019/8030697

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Copyright © 2019 Min Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

The meiotic genes were expressed in germ cells of both male and female Wt1^-/flox; Cre-ER^TM mice after tamoxifen induction. Wt1^flox/flox females were crossed with Wt1^-/flox; Cre-ER^TM mice, and the pregnant females were injected with tamoxifen at E9.5 to induce Cre activity. Wt1^flox/flox and Wt1^-/flox embryos were used as controls. A–H: immunofluorescence analysis of STRA8/MVH (A–D) and SYCP3/MVH (E–H) in control and Wt1^-/flox; Cre-ER^TM embryos at E13.5. Germ cells were labeled with MVH. DAPI (blue) was used to stain the nuclei. The arrows point to double-positive germ cells. I: real-time PCR analysis of Stra8, Sycp3, Dmc1, and Rec8 in control and Wt1^-/flox; Cre-ER^TM embryos at E13.5. Hprt1 was used as an endogenous control. The data are presented as mean ± SEM. ^∗P < 0.05; ^∗∗P < 0.01.