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. 2019 Sep 5;2019:8030697. doi: 10.1155/2019/8030697

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Copyright © 2019 Min Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The meiosis was blocked in germ cells of Wt1^-/flox; Cre-ER^TM gonads. Wt1^flox/flox females were crossed with Wt1^-/flox; Cre-ER^TM mice, and the pregnant females were injected with tamoxifen at E9.5 to induce Cre activity. Control (Wt1^flox/flox and Wt1^-/flox) and Wt1^-/flox; Cre-ER^TM gonads were dissected at E13.5 and cultured in vitro for 3 days. MVH/SYCP3 (A–D) and MVH/γH2AX (E–H) double-staining experiment was performed, and germ cells were labeled with MVH (green). DAPI (blue) was used to stain the nuclei. The gender of the embryos was confirmed with PCR using Sry primers.