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. 2019 Sep 10;9:317. doi: 10.3389/fcimb.2019.00317

Figure 2.

Figure 2

NDV infection increases the expression of SOCS3. (A) CEF cells were infected with NDV (0.1 MOI). IFA was performed to detect the expression of SOCS3 and NDV 24 hpi. (B,C) CEF cells were infected with NDV (0.1 MOI), cells were harvested and qRT-PCR and WB were performed to test the expression of SOCS3 at different times. (D) WB was performed to detect the protein expression of SOCS3 at 24 h post different MOI NDV (0.01 MOI, 0.1 MOI, 1 MOI) infection. (E,F) CEF cells were infected with different virulence NDV. At 24 hpi, cells were harvested and qRT-PCR and WB were performed to test the expression of SOCS3. Data presented are from three independent experiments and presented as mean ± SD. *P < 0.05; **P < 0.01.