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. 2019 Sep 10;9:317. doi: 10.3389/fcimb.2019.00317

Figure 5.

Figure 5

Knock-down of SOCS3 inhibits NDV replication. (A,B) CEF cells were co-transfected with a pCMV-SOCS3-HA expression plasmid and non-targeting control siRNA (NC) or SOCS3-targeting siRNAs, followed by qRT-PCR and WB using an anti-HA antibody to compare the efficiencies of these three siRNAs. (C,D) CEF cells were transfected with NC or #3. CEF cells were infected NDV (0.1 MOI) 24 h post transfection, and cells and supernatant were harvested at 12 and 24 hpi. WB was performed to detect the expression of HN protein of NDV (C). TCID50 was used to measure viral RNA replication (D). (E) WB was performed to detect the expression of STAT1 and phosphorylated STAT1 in SOCS3-knockdown cells at 24 hpi. (F,G) qRT-PCR was used to detect the expression of MX1 and OASL. Data presented are from three independent experiments and presented as mean ± SD. *P < 0.5, **P < 0.01.