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. 2019 Sep 10;9:317. doi: 10.3389/fcimb.2019.00317

Figure 6.

Figure 6

MEK/ERK signaling pathway is involved in the expression of SOCS3. (A) phosphorylated ERK1/2 was detected after NDV (0.1 MOI) infection at different time. (B) CEF cells were treated with 20 μM of the ERK 1/2 inhibitor U0126 or DMSO for 24 and 48 h, and CCK-8 assay was performed to analyze the cytotoxicity of U0126 (20 μM) to CEF cells. CEF cells were treated with 20 μM of the ERK 1/2 inhibitor U0126 or DMSO for 12 h and infected with NDV (0.1 MOI). Cells were harvested at 24 hpi. Phosphorylated ERK1/2 was detected by WB (C), and qRT-PCR (D) and WB (E) were used to detect the expression of SOCS3. (F,G) CEF cells were treated with 20 μM of the ERK 1/2 inhibitor U0126 or DMSO for 12 h and then transfected with a pCAGGS-V-Flag (V) expression plasmid or pCAGGS-Flag (Vector). Cells were harvested 24 h post transfection. qRT-PCR (F) and WB (G) were used to detect the expression of SOCS3. Data presented are from three independent experiments and presented as mean ± SD. **P < 0.01.