Table 1.
Primers and siRNA sequence information.
| Name | Forward primer (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|
| SOCS3 (clone) |
CCGGAATTC ATGGTCACCCACAGCAAGTTC |
GGTAGATCT TTAGAGGGGGGCATCGTACTG |
| IFN-α (qRT-PCR) |
GACATGGCTCCCACACTACC | AGGCGCTGTAATCGTTGTCT |
| IFN-β (qRT-PCR) |
GCTCACCTCAGCATCAACAA | GGGTGTTGAGACGTTTGGAT |
| OASL (qRT-PCR) |
AGATGTTGAAGCCGAAGTACCC | CTGAAGTCCTCCCTGCCTGT |
| MX1 (qRT-PCR) |
AAGCCTGAGCATGAGCAGAA | TCTCAGGCTGTCAACAAGATCAA |
| NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGA ATT |
| siSOCS3 #1 (218) |
GCUCUAAGAGCGAGUACCATT | UGGUACUCGCUCUUAGAGCTT |
| siSOCS3 #2 (576) |
CAAACGCACCUACUACAUUTT | AAUGUAGUAGGUGCGUUUGTT |
| siSOCS3 #3(697) |
GACUCCUACGAGAAGAUGATT | UCAUCUUCUCGUAGGAGUCTT |