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. 2017 Jun 19;10(5):361–366. doi: 10.1080/21541248.2017.1327838

Figure 2.

Figure 2.

Effect of the FHL family on PLEKHG2-induced SRE-dependent gene transcription and the interaction with PLEKHG2. (A) HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, Myc-tagged PLEKHG2 and Flag-tagged FHL1A, FHL2a or FHL3-1, as indicated. Transfected cells were lysed 24 h after transfection, and the effects of FHL1A, FHL2a and FHL3-1 on PLEKHG2-induced transcription were analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± SD (error bars). The data shown are representative of three independent experiments. (B) HEK293 cells were co-transfected with Myc-tagged PLEKHG2 and Flag-tagged FHL1A, FHL2a or FHL3-1, as indicated. Transfected cells were lysed 24 h after transfection, and PLEKHG2 was immunoprecipitated with anti-Myc antibody. Precipitated proteins were separated by SDS-PAGE and immunoblotted with anti-Myc (for PLEKHG2) antibody and anti-FLAG antibody (for FHL1A, FHL2a and FHL3-1). TCL, total cell lysate; IP, immunoprecipitation; IB, immunoblotting. The data shown are representative of three independent experiments.