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. 2017 Sep 26;10(6):466–484. doi: 10.1080/21541248.2017.1339767

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© 2017 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The effects of single knockdowns of RhoA RhoB and RhoC on basal endothelial morphology. (A) Immunofluorescent staining of VE-cadherin (green), F-actin (white) and nuclei (blue) in HUVECs for visualization of adherens junctions following loss of RhoA, RhoB and RhoC. Scale bars represent 50 μM. Representative pictures of 3-4 experiments. (B) VE-cadherin Integrated Density/cell; each point representing an individual measurement. (C) VE-cadherin area per cell. (D) F-actin Integrated Density per cell. (E) Western blot analysis for VE-cadherin of whole cell lysates collected from HUVECs 72 hours after transfection with siNT, siRhoA, siRhoB or siRhoC. Representative blots of 3 experiments are shown. Tubulin is included as loading control. Bar graphs represent mean ± SEM from 3 individual experiments all normalized to siNT. Data in panel B,C and D represent mean ± SEM (bar graphs) of 12–16 values from 4 independent experiments. *p < 0.05, ***p < 0.001, ****p < 0.0001 in Dunnett's post-hoc analysis of one-way ANOVA.