Figure 5.

Ribosome display library synthesis and downstream assays for high-throughput identification of protein ligands and interaction partners. a) Workflow for co-translational nucleic acid-barcoding of proteins via ribosome display and subsequent screening for protein-binding partners of an immobilized antigen of interest. Isolated mRNA from ‘hits’ can be identified via NGS or subjected to additional rounds of library generation and screening. b) The single molecule interaction-sequencing (‘SMI-seq’) method developed by the Church lab.