Table 1. Bacterial strains and plasmids.
| Strains | Genotype | Resistancea | Reference |
|---|---|---|---|
| E. coli | |||
| DH5α | F−endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ– | Life Science Technologies | |
| C43(DE3) | F−ompT gal dcm hsdSB(rB- mB-)(DE3) | Avidis | |
| BWPGPTs | BW25113 ΔpgpA ΔpgpC ΔpgpB::Cm + pMAKkanpgpB | Cm, Kan | [6] |
| BWTsbacA | BW25113 ΔbacA ΔybjG ΔpgpB::Kan + pMAKbacA | Cm, Kan | [7] |
| H. pylori | |||
| HP-1 | N6 | [20] | |
| HP-2 | N6 lpxE::Gm | Genta | This work |
| HP-3 | N6 hp0350::Km | Kan | This work |
| HP-4 | N6 lpxF::Km | Kan | This work |
| HP-13 | N6 hupA::Km | Kan | This work |
| HP-5 | N6 pILL2150 | Cm | This work |
| HP-7 | N6 pILL2150 bacA | Cm | This work |
| HP-8 | N6 pILL2150 lpxT | Cm | This work |
| HP-9 | N6 pILL2150 pgpB | Cm | This work |
| HP-10 | N6 pILL2150 ybjG | Cm | This work |
| HP-14 | N6 pILL2150 lpxE | Cm | This work |
| HP-15 | N6 pILL2150 hp0350 | Cm | This work |
| HP-16 | N6 pILL2150 lpxF | Cm | This work |
| N6 +pILL2157 bacA | N6 pILL2157 bacA | Cm | This work |
| N6 +pILL2157 lpxT | N6 pILL2157 lpxT | Cm | This work |
| N6 +pILL2157 pgpB | N6 pILL2157 pgpB | Cm | This work |
| N6 +pILL2157 ybjG | N6 pILL2157 ybjG | Cm | This work |
| HP-22 | X47-2AL | [21] | |
| HP-50 | X47 hupA::Km | Kan | This work |
| HP-37 | N6 hupA::Km pILL2150 lpxE | Kan, Cm | This work |
| HP-38 | N6 hupA::Km pILL2150 hp0350 | Kan, Cm | This work |
| HP-39 | N6 hupA::Km pILL2150 hupA | Kan, Cm | This work |
| HP-40 | N6 hupA::Km pILL2150 lpxF | Kan, Cm | This work |
| HP-33 | N6 hupA::Km pILL2150 | Kan, Cm | This work |
| HP-49 | N6 pgpA::Km | Kan | This work |
| Plasmids | |||
| Topo TAΔlpxE:Gm | Genta | This work | |
| Topo TAΔlpxE:Km | Kan | This work | |
| Topo TAΔhp0350:Km | Kan | This work | |
| Topo TAΔhupA:Km | Kan | This work | |
| Topo TAΔlpxF:Km | Kan | This work | |
| pTrcHis30 lpxE | Amp | This work | |
| pTrcHis30 hp0350 | Amp | This work | |
| pTrcHis30 hupA | Amp | This work | |
| pTrcHis30 lpxF | Amp | This work | |
| pTrcHis30 pgpA | Amp | This work | |
| pILL2150 lpxE | Cm | This work | |
| pILL2150 hp0350 | Cm | This work | |
| pILL2150 hupA | Cm | This work | |
| pILL2150 lpxF | Cm | This work | |
| pILL2150 bacA | Cm | This work | |
| pILL2150 lpxT | Cm | This work | |
| pILL2150 pgpB | Cm | This work | |
| pILL2150 ybjG | Cm | This work | |
| pILL2157 bacA | Cm | This work | |
| pILL2157 lpxT | Cm | This work | |
| pILL2157 pgpB | Cm | This work | |
| pILL2157 ybjG | Cm | This work |
(a) Genta or Gm, Kan or Km, Cm and Amp: gentamycin, kanamycin, chloramphenicol and ampicillin resistance cassettes, respectively.