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. 2019 Sep 5;15(9):e1007972. doi: 10.1371/journal.ppat.1007972

Table 3. Functional complementation of E. coli BWTsbacA conditional strain by PAP2 encoding genes from H. pylori.

E. coli BWTsbacA CFU/mL
30°C 42°C 30°C
+IPTG 1mM		 42°C
+IPTG 1mM
- 782 0 876 1
+pTrc His30 lpxE 229 222 0 0
+pTrc His30 hp0350 396 2 354 0
+pTrc His30 hupA 265 219 186 172
+pTrc His30 lpxF 180 0 0 0

The E. coli thermosensitive strain was transformed with the pTrcHis30-based plasmids and aliquots were plated onto two ampicillin-containing 2YT agar plates incubated at either 30°C or 42°C. The CFU were counted after 24 h incubation.