Table 4. Complementation of lpxE/hupA double mutant with an ectopic copy of H. pylori PAP2 encoding genes.
|
Helicobacter pylori N6 hupA::Km |
Transformation rate (transformants/cfu/μg TopoTAΔlpxE) |
|
|---|---|---|
| -ITPG | +IPTG (1mM) | |
| +pILL2150 empty | 0 | 0 |
| +pILL2150 lpxE | 0 | 1.44E-05 |
| +pILL2150 hp0350 | 0 | 0 |
| +pILL2150 hupA | 0 | 1.16E-05 |
| +pILL2150 lpxF | 0 | 0 |
Quantification of lpxE gene inactivation in ΔhupA single mutant transformed with plasmids expressing one PAP2 from H. pylori. The transformation rates were measured in the presence and in the absence of IPTG.