Table 6. Complementation of E. coli BWPGPTs conditional strain by PAP2 and PgpA encoding genes from H. pylori.

E. coli BWPGPTs	CFU/mL
	30°C	42°C
-	211	1
+pTrc His30 lpxE	97	140
+pTrc His30 hp0350	152	0
+pTrc His30 hupA	289	279
+pTrc His30 lpxF	75	0
+pTrc His30 pgpA	652	844

The E. coli thermosensitive strain was transformed with the pTrcHis30-based plasmids and aliquots were plated onto two ampicillin-containing 2YT agar plates incubated at either 30°C or 42°C. The CFU were counted after 24 h incubation.