Table 8. Complementation of lpxE/hupA double mutant with an ectopic copy of E. coli PAP2/BacA encoding genes.

Helicobacter pylori N6 hupA::Km	Transformation rate (transformants/cfu/μg TopoTAΔlpxE)
	-ITPG	+IPTG (1mM)
+pILL2150 empty	0	0
+pILL2150 bacA	0	0
+pILL2150 lpxT	0	0
+pILL2150 pgpB	0	1.08E-05
+pILL2150 ybjG	0	0
+pILL2157 bacA	3.85E-05	5.25E-05
+pILL2157 lpxT
+pILL2157 pgpB	1.51E-05	2.07E-05
+pILL21570 ybjG	5.86E-06	1.06E-06

Quantification of lpxE gene inactivation in ΔhupA single mutant transformed with plasmids expressing one PAP2/BacA from E. coli. Two types of expression vectors were used: pILL2150 and pILL2157. The pILL2150 vector possesses an E. coli promoter and leads to low levels of expression in H. pylori, while pILL2157 displays a H. pylori promoter yielding high levels of expression in H. pylori. The transformation rates were measured in the presence and in the absence of IPTG.