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. 2019 Apr 6;24(1):27–33. doi: 10.1080/13510002.2019.1601448

Figure 2.

Figure 2.

Effect of antioxidant NAC supplementation on ROS and autophagy markers in the lungs of mice exposed to cigarette smoke (CS) for 45 days (n = 10 per group). Dichlorofluorescein (DCF) level (a), protein levels of SESN2 (b), AMPK phosphorylation (c), mTOR phosphorylation (d), ULK1 phosphorylation (e), ATG12 (f), LC3B (g), and representative western blot of the considered proteins (h) in the control (non-exposed group), control plus NAC, CS exposure for 45 days without NAC, and 45 days of CS exposure with NAC. Data are expressed as the mean and standard error of the means (mean ± SEM) and analyzed statistically by one-way analysis of variance, followed by Tukey’s HSD post hoc test. (*p < 0.05 versus control and control + NAC; #p < 0.05 versus 45 + NAC).