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. 2019 Jun 14;11(6):1064–1076. doi: 10.1080/19420862.2019.1624127

Figure 2.

Figure 2.

Reduction time-course data. Red, blue, and green represent three different reduction conditions. (A) GX data. H, heavy chain; L, light chain; HL, combination of heavy chain-light chain; HH, combination of heavy chain-heavy chain; HHL, combination of heavy chain-heavy chain-light chain; HHLL, heavy chain-heavy chain-light chain-light chain combination (whole antibody). (B) Nonreduced peptide map data. H15 is the hinge peptide containing three cysteines: two that form interchain disulfide bonds between the two heavy chains, and another engineered cysteine designed for conjugation. H14 and L16 are peptides containing cysteines that connect the antibody heavy chain and light chain. H15 (3SH) and L16 (1SH) are the final products of the reduction reaction. L16-H14 (1SS), peptide structure with disulfide bond link heavy chain and light chain; H15-H15 (2SS, 2Cys), peptide structure with disulfide bonds linking two heavy chains and two inserted cysteine capped with cysteinylation in the starting material; H15-H15 (3SS), structure with three disulfide bonds between two H15 peptides; H15-H15 (2SS, 1Cys, 1SH), intermediate with cysteinylation removed from one inserted cysteine; H15-H15 (2SS, 2SH), intermediate with cysteinylation removed from both inserted cysteines; H15 (1SS, 1SH), half-antibody structure intermediate.