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. 2019 Jun 14;11(6):1064–1076. doi: 10.1080/19420862.2019.1624127

Figure 3.

Figure 3.

Oxidation time-course data. Red and blue represent two different oxidation conditions. (A) GX data. H, heavy chain; L, light chain; HL, combination of heavy chain-light chain; HH, combination of heavy chain-heavy chain; HHL, combination of heavy chain-heavy chain-light chain; HHLL, heavy chain-heavy chain-light chain-light chain. (B) Nonreduced peptide map data. H15 is the hinge peptide containing three cysteines: two that form interchain disulfide bonds between the two heavy chains, and another engineered cysteine designed for conjugation. H14 and L16 are peptides containing cysteines that connect the antibody heavy chain and light chain. H15 (3SH) and L16 (1SH) are the starting products for the oxidation reaction. H15-H15 (2SS, 2SH) and L16-H14 (1SS) are the desired final products of the oxidation reaction and contain disulfide bonds between the two heavy chains (H15-H15 (2SS, 2SH)) or the heavy and light chain (L16-H14 (1SS)). H15-H15 (1SS, 4SH), intermediate with one disulfide bond between two H15 peptide and four free cysteine; H15-H15 (3SS), undesired product containing three disulfide bonds, which correspond to the unconjugated antibody in the final ADC product; H15 (1SS, 1SH), undesired product corresponding to half-antibody, which is converted to half-ADC in the final ADC product.