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. 2019 Jun 14;11(6):1064–1076. doi: 10.1080/19420862.2019.1624127

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Conjugation time-course data. Red and blue represent two different conjugation conditions. (A) GX data. H, heavy chain; L, light chain; HL, combination of heavy chain-light chain; HHL, combination of heavy chain-heavy chain-light chain; HHLL, heavy chain-heavy chain-light chain-light chain. (B) Nonreduced peptide map data. H15 is the hinge peptide containing three cysteines: two that form interchain disulfide bonds between the two heavy chains, and another engineered cysteine designed for conjugation. H14 and L16 are peptides containing cysteines that connect the antibody heavy chain and light chain. H15-H15 (2SS, 2SH) is the desired starting product for conjugation, and H15 (1SS, 1SH) is the undesired, half-antibody starting product. H15-H15 (2SS, 2Drug) is the desired final product of conjugation. H15-H15 (2SS, 1SH, 1Drug), underconjugated product with a DAR of 1; H15 (1SS, 1Drug), half-ADC; H15-H15 (3SS), triple-disulfide bond structure (unconjugated antibody) generated during the oxidation step; H14 (1Drug), undesired overconjugated heavy-chain species; L16 (1Drug), undesired overconjugated light-chain species.