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. 2019 Jun 14;11(6):1064–1076. doi: 10.1080/19420862.2019.1624127

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Scheme II. — Disulfide bond exchange reaction. The free thiol of quenching reagent reacts with disulfide bond in ADC, which breaks the disulfide bond in ADC and attaches quenching reagent to heavy chain or light chain.