Table 1.
Intact mass and nonreduced peptide map data for HIC fractions.
| HIC PK# | Intact Structure | Intact Mass (Da) |
Peptide Name | Peptide Structure |
Peptide Mass (Da) |
||
|---|---|---|---|---|---|---|---|
| Theor | Exper | Theor | Exper | ||||
| 1 |
 Unconjugated/Tri SS bonds |
146,242 | 146,242 | H15-H15 (3SS) |  |
5661.8 (a) | 5661.9 (a) |
| 2 |
 DAR 1 ADC |
147,741 | 147,742 | H15-H15 (2SS, 1Drug, 1SH) |  |
7285.6 (a) | 7285.8 (a) |
| 3 |
 (S,S), (R,S), (R,R) Designed DAR2 ADC |
149,237 | 149,236 | H15-H15 (2SS, 2Drug) |  |
8657.2 (a) | 8657.5 (a) |
| 4 | 149,237 | 8657.5 (a) | |||||
| 5 | 149,234 | 8657.5 (a) | |||||
| 6 |
 DAR3 ADC (a) |
150,734 | 150,748 | H14-Drug |  |
1946.9 (m) | 1946.9 (m) |
 DAR3 ADC (b) |
L16-Drug |  |
2307.0 (m) | 2307.1 (m) | |||
 LC-Drug |
25,116 | 25,115 | |||||
 DAR4 ADC |
152,231 | 152,249 | L16-H14 (1SS) |  |
1260.5 (m) | 1260.5 (m) | |
| Crossing HIC peaks |
 Half ADC |
74,619 | 74,620 | H15 (1SS, 1Drug) |  |
4326.1 (m) | 4326.2 (m) |
 LC-QuechCap |
23,781 | 23,780 | L16-QC (Quench Cap) |  |
972.3 (m) | 972.4 (m) | |
(a) Average mass; (m) mono isotopic mass.