Table 1.

N-glycan compositions and proposed structures with retention times (RT) identified on N-glycosylation site Asn163 of atacicept. Samples were treated with pronase and glycopeptides were analyzed by C18-PGC-LC-ESI-MS/MS. Hex = white circle = hexose, HexNAc = N-acetylhexosamine, Fuc = red triangle = fucoses, NeuAc = purple diamond = N-acetylneuraminic acid, yellow circle = galactose, blue square = N-acetylglucosamine, green circle = mannose.

Monosaccharide composition	RT [min]	Proposed structure	Monosaccharide composition	RT [min]	Proposed structure
HexNAc2Hex5	40.1		HexNAc4Hex3	37.4
HexNAc2Hex6	38.6		HexNAc4Hex3Fuc1	39
HexNAc2Hex6	40.8		HexNAc4Hex4	38.3
HexNAc2Hex7	38.6		HexNAc4Hex4Fuc1	39.8
HexNAc2Hex7	40.4		HexNAc4Hex4Fuc1 NeuAc1	10.3/51.6
HexNAc2Hex8	39.8		HexNAc4Hex5	39.1
HexNAc2Hex10	43.6		HexNAc4Hex5Fuc1	40.5
HexNAc3Hex3	37.2		HexNAc4Hex5 NeuAc1	51.2
HexNAc3Hex3Fuc1	39		HexNAc4Hex5Fuc1 NeuAc1	10.3/51.6
HexNAc3Hex3Fuc1	39.9		HexNAc4Hex5Fuc1 NeuGc1	51.2
HexNAc3Hex3Fuc1	41.1		HexNAc4Hex5Fuc1 NeuAc2	11.6
HexNAc3Hex4	38.2		HexNAc4Hex5Fuc2 NeuAc2	11.4
HexNAc3Hex4Fuc1	39.7		HexNAc4Hex5Fuc1 NeuAc1NeuGc1	11.4
HexNAc3Hex4Fuc1	40.4		HexNAc5Hex3Fuc1	39.2
HexNAc3Hex4Fuc1	41.8		HexNAc5Hex3Fuc1	40.2
HexNAc3Hex4Fuc1 NeuAc1	51.6		HexNAc5Hex4Fuc1	39.9
HexNAc3Hex5Fuc1	38.6		HexNAc5Hex4Fuc1	40.8
HexNAc3Hex5Fuc1	39.2		HexNAc5Hex5Fuc1	40.4
HexNAc3Hex5Fuc1	40.4		HexNAc5Hex5Fuc1	41.2
HexNAc3Hex6Fuc1	39.4		HexNAc5Hex6Fuc1	41.6
HexNAc3Hex6Fuc1	40.4		HexNAc5Hex6Fuc1	42.5
HexNAc4Hex6Fuc1	41.4		HexNAc5Hex6Fuc1 NeuAc1	10.6
HexNAc3Hex5Fuc1 NeuAc1	51.4		HexNAc5Hex6Fuc1 NeuAc2	11.1
HexNAc3Hex6Fuc1 NeuAc1	51.7