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. 2019 Jul 16;11(7):1206–1218. doi: 10.1080/19420862.2019.1632113

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Phosphorylation pattern analysis of FcϵR1 subunits.

a) Assessment of phosphorylation patterns of β subunits after stimulation with DNP-BSA is shown using three antibodies p1-1, p13B1, and p3p10 utilizing western blotting with antibodies in minibody format. The FcϵR1 receptor was stimulated with 2 nM DNP-BSA and the data show phosphorylation patterns at various time points post stimulation. Phosphorylation at resting (basal) is also given. Antibody Z3 and rat kidney cell lysate (RK) were used as negative controls. The lower panel shows the total β detection used as a loading control. b) Similar analysis performed for anti-γ PV1.3. c) Patterns of phosphorylation graphed with data from multiple experiments (3–8 cell lysates prepared at different times).