Table 4.

Four sets of selection strategy is shown using p1 peptide selection as an example. The four selection strategies are 1) no competition with acid elution 2) competition with 10X excess of non-specific peptides with acid elution 3) no competition, but elution by 10X excess of specific peptide 4) both competition and specific elution strategies were combined.

	antigen	competition	elution
Set 1	Bio-p1 1μM		0.1M HCl
Set 2	Bio-p1 1μM	10μM nb-p0 + 10μM nb-p2 + 10μM nb-p3 + 10μM nb-p23	0.1M HCl
Set 3	Bio-p1 1μM		10μM nb-p1
Set 4	Bio-p1 1μM	10μM nb-p0 + 10μM nb-p2 + 10μM nb-p3 + 10μM nb-p23	10μM nb-p1