Figure 1.

Ionic strength dependence of mAb1/HSA and mAb2/HSA cross-interactions measured by CG-MALS. Cross-virial coefficients (A₂₃) were determined by CG-MALS for interactions between 10 g/L HSA and 10 g/L mAb1 (red) or mAb2 (blue) at increasing concentrations of NaCl in phosphate buffer. Negative values for CVC indicate attractive forces between the molecules, while positive CVC values indicate repulsive forces between the molecules. The box indicates physiological ionic strength where the CVC values for mAb1 and mAb2 are negative and positive, respectively.