Skip to main content
. 2019 Mar 25;24(1):17–26. doi: 10.1080/13510002.2019.1596431

Figure 1.

Figure 1.

Reactive oxygen species production is attenuated in hepatocytes expressing HCV Core or NS3/4A proteins and under external oxidative stress induction. Total reactive oxygen species (a) and mitochondrial superoxide anion production (b) were detected using a set of fluorogenic probes (Cell ROX® Deep Red Reagent and MitoSOX™ Red Reagent, respectively) in Huh-7 cells transiently transfected with the empty vector, pTracerCore or pTracerNS3/4A. Cells were treated with menadione (50 μM) 24 hours post-transfection for 6 hours. As a control and to inhibit the effect of menadione, cells were pre-treated 30 minutes prior to menadione treatment with the anti-oxidant NAC (5 mM). 2-way ANOVA was performed for group comparisons of the means with a Bonferroni post-test and in specific cases a t test was applied to compare the means between single comparisons; the asterisks represent the p value as: ***<0.001, **<0.003 and, *<0.05. (p value > 0.05). NT = No treated cells. n.s. = not significant. Experiments were conducted in duplicate wells and results are expressed as the average of three independent experiments.