Table 1.

Survey of antibody pairs to investigate BsIgG₁ yields without Fab mutations but with KIH HC mutations for HC heterodimerization. Different combinations of IgG₁ HC and LC pairs were co-transfected into Expi293F cells and the yield of BsIgG₁ determined by high-resolution LCMS following protein A chromatography and SEC. The yield of correctly assembled BsIgG₁ in isobaric mixtures that also contained LC-scrambled IgG₁ (see Figure 1 for representative examples) was estimated using a previously developed algebraic formula.²² Data shown are the yield of BsIgG₁ from optimized LC DNA ratios. BsIgG₁ yields >65% are indicated in bold. The HC of mAb-1 contained the ‘hole’ mutations (T366S:S368A:Y407V)⁸ and the HC for mAb-2 contained a ‘knob’ mutation (T366W).⁸ NA, not applicable: monospecific antibodies.