Figure 5.

The process for creating bispecific antibodies with asymmetric deamidation at Asn330 is presented. (a) mAb1 (blue) and mAb2 (peach) were stressed at 40°C at pH 5.5 to induce selective deamidation at the Asn330 residue in the Fc Region (brown with red lines). Combinations of native or stressed mAb1 and mAb2 were pooled prior to Fab-arm exchange to generate control BsAb5, asymmetrically deamidated BsAb6 and BsAb7, and symmetrically deamidated BsAb8. (b) The bar plot illustrates the levels of FcγRIIIa binding of BsAb6, BsAb7, and BsAb8 relative to the control BsAb5 sample. The HC Asn330 deamidation values for the mAb1 and mAb2 arms of each BsAb are shown above the corresponding bar.