Table 3.

Binding affinities K_D (M) of representative anti-SIRPα antibodies. Representative clones from each epitope bin were selected and their binding affinities to various SIRP (human SIRPα v1, v2; cynomolgus SIRPα; mouse NOD, BL6, BALB/c SIRPα; human SIRPγ; human SIRPβ1) were determined by SPR. A Langmuir kinetic model was used for fitting and calculation of K_D (M). The ability of the antibody to block CD47 binding to SIRPα is indicated in the last column.

Bin		Antibody1	Source4	V1	V2	Cyno	NOD	BL6	BALB/c	SIRPγ	SIRPβ1	CD47 Blocking
1		119	H	1.83E-10	6.82E-11	1.12E-10	NB2	NB	NB	2.67E-10	3.42E-10	Block
1		135	H	1.51E-10	2.90E-11	9.69E-11	NB	NB	NB	5.39E-10	1.10E-10	Block
1		21	S	<1.00E-12	<1.00E-12	3.61E-12	5.47E-10	4.60E-10	1.05E-09	<1.00E-12	<1.00E-12	Block
2		115	H	4,26E-10	1.86E-09	2.41E-09	NB	NB	NB	B3	B	Kick-off
3		136	H	4.58E-10	1.63E-09	2.15E-09	5.54E-10	1.27E-08	3.50E-10	2.39E-08	4.35E-09	Non-block
4		3	W	1.62E-10	7.67E-11	2.29E-09	1.63E-09	3.65E-09	1.16E-09	8.36E-08	1.63E-09	Non-Block
4		173	H	9.37E-10	9.28E-09	4.46E-08	NB	NB	NB	NB	B	Non-Block
4		209	S	1.71E-10	5.01E-09	3.90E-08	NB	NB	NB	8.99E-09	B	Non-Block
4		213	S	6.05E-09	1.69E-09	4.49E-08	NB	NB	NB	2.02E-09	1.71E-08	Non-Block
5		123	H	6.05E-10	NB	1.62E-09	NB	NB	NB	7.47E-10	7.07E-08	Non-Block
5		149	H	8.73E-10	2.38E-10	7.64E-09	NB	NB	NB	1.89E-09	2.06E-10	Non-Block
5		161	H	1.03E-09	1.27E-10	6.35E-09	NB	NB	NB	2.84E-09	2.63E-09	Non-Block
5		162	H	4.50E-10	1.57E-08	1.26E-08	NB	NB	NB	3.97E-09	3.00E-09	Non-Block
5		194	H	4.97E-10	NB	9.11E-10	NB	NB	NB	9.47E-10	5.36E-08	Non-Block
5		218	S	1.23E-10	2.76E-10	5.99E-11	NB	NB	NB	6.36E-11	B	Non-Block
6		45	W	6.63E-11	1.34E-10	NB	NB	NB	NB	2.71E-08	1.06E-08	Non-Block

1. All antibodies are screened as full IgG1 except 173, 209, 213, 123, 149, 161, 162, 194, 218 are screened as scFv-Fc.

2. NB = no binding

3. B = binding confirmed and exact K_D value was not determined.

4. W = wt, H = OmniChicken, S = SynVH chickens.