Figure 4.

Effects of LBL-007 on regulation of activated T cells and increase of IL-2 production.

(A,B) NFAT reporter assay was used to reveal LBL-007 binding to Jurkat-NFAT-LAG-3 cells. The corresponding fluorescence intensity of serial dilutions and EC50 was detected. After activating NFAT, Supernatants were collected and IL-2 levels were measured using the Human IL-2 DuoSet ELISA Kit. Data are expressed as the mean ± SD on triplicates. (C) A series of diluted concentrations of antibodies binding to activated human T cells was assessed by flow cytometry. Data are showed as Mean Fluorescent Intensity and EC50. (D) Different concentrations of LBL-007, relatlimab analog, and IgG4 were incubated with human PBMCs for 3 days, the IL-2 level in the supernatants was measured using ELISA Kit. Data are expressed as the mean ± SD on triplicates. *P < .05 and **P < .01 by Student’s t-test.