KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-GFP | Invitrogen | Cat # A10262 |
| Rat monoclonal anti-N-cadherin | Developmental Studies Hybridoma Bank | Cat # DN-Ex #8; RRID AB_528121 |
| Guinea pig polyclonal anti-Sidekick | Astigarraga et al., 2018 | N/A |
| Rat monoclonal anti-E-cadherin | Developmental Studies Hybridoma Bank | Cat # DCAD2; RRID AB_528120 |
| Mouse monoclonal anti-Gliotactin | Auld et al., 1995 | RRID AB_2566915 |
| Rabbit monoclonal anti-myosin light chain 2 (Ser19) | Cell Signaling | Cat # 3675; RRID AB_2250969 |
| Mouse monoclonal anti-actin | MP Biomedicals | Clone C4; RRID AB_2335127 |
| Rabbit polyclonal anti-β-galactosidase | MP Biomedicals | Cat # 0855976; AB_2334934 |
| Rabbit polyclonal anti-Canoe | Speicher et al., 2008 | N/A |
| Mouse monoclonal anti-Discs large | Developmental Studies Hybridoma Bank | Cat # 4F3; RRID AB_528203 |
| Mouse monoclonal anti-Polychaetoid | Developmental Studies Hybridoma Bank | Cat # PYD2; RRID AB_2618043 |
| Rabbit polyclonal anti-Zipper | Tomokazu Ohshiro | N/A |
| Rat monoclonal anti-HA 3F10 | Roche | Cat # 11815016001; RRID AB_390914 |
| Mouse monoclonal anti-Myc tag 9B11 | Cell Signaling | Cat # 2040; RRID AB_2148465 |
| GFP-Trap A | Chromotek | Cat # gta-10; RRID AB_2631357 |
| Alexa Fluor 555-conjugated phalloidin | Invitrogen | Cat # A34055 |
| Guinea pig anti-Sqh1P | Zhang and Ward, 2011 | N/A |
| Rabbit polyclonal anti-GFP | Molecular Probes - Thermo Fisher Scientific | Cat # A11122; RRID: AB_221569 |
| Bacterial and Virus Strains | ||
| E.coli BL21 | Sigma | Cat # B2685-10X50μl |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Effectene Transfection Reagent | Qiagen | Cat # 301425 |
| Dextran, Rhodamine B, 10,000MW, Neutral | ThermoFisher Scientific | Cat # 1824 |
| Gibco® Schneider’s Drosophila medium | Invitrogen | Cat # 21720001 |
| Gibco® Fetal bovine serum, qualified, heat inactivated | Invitrogen | Cat # 16140-071 |
| Shields and Sang M3 insect medium | Sigma | Cat # S3652 |
| Latrunculin A | Enzo | Cat # BML-T119 |
| Octane | Sigma-Aldrich | Cat # 657042 |
| GST-Sdk | This paper | N/A |
| GST-SdkΔBD | This paper | N/A |
| cOmplete Protease Inhibitor Cocktail | Roche | Cat # 11873580001 |
| Glutathione-sepharose beads | Thermo-Fisher | Cat # 17075601 |
| 10-S Voltalef oil | VWR Chemicals | Cat # 24627.188 |
| Experimental Models: Cell Lines | ||
| D. melanogaster: Cell line S2: S2-DRSC | Laboratory of Ruth Lehmann | FlyBase: FBtc0000181 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: sdkMB05054 | Bloomington Drosophila Stock Center | BDSC; 24603; Flybase: FBal0198242 |
| D. melanogaster: sdkΔ15 | Astigarraga et al., 2018 | BDSC; 24603; Flybase: FBal0340184 |
| D. melanogaster: sdkCPTI00037 | Kyoto Drosophila Genomics and Genetic Resources | Kyoto; 115107; Flybase: FBal0262454 |
| D. melanogaster: sdkMI101498-GFSTF.1 | Bloomington Drosophila Stock Center | BDSC; 60169; Flybase; FBti0178565 |
| D. melanogaster: akaYD1046 | Bloomington Drosophila Stock Center | BDSC; 51179; Flybase: FBal0212250 |
| D. melanogaster: pydMI01205-GFSTF.1 | Bloomington Drosophila Stock Center | BDSC; 60166; Flybase: FBal0314561 |
| D. melanogaster: UAS-LifeAct-Ruby | Bloomington Drosophila Stock Center | BDSC; 35545; Flybase: FBti0143328 |
| D. melanogaster: P{ubi-GFP.D} | Bloomington Drosophila Stock Center | BDSC; 1681; Flybase: FBti0004735 |
| D. melanogaster: longGMR-GAL4 | Bloomington Drosophila Stock Center | BDSC; 8605; Flybase: FBti0058798 |
| D. melanogaster: C381-GAL4 | Manseau et al., 1997 | N/A |
| D. melanogaster: UAS-MbsN300 | Lee and Treisman, 2004 | Flybase: FBal0182792 |
| D. melanogaster: UAS-MLCK.ct | Bloomington Drosophila Stock Center | BDSC; 37527; Flybase: FBti0026705 |
| D. melanogaster: sqhAX3 | Bloomington Drosophila Stock Center | BDSC; 25712; Flybase: FBal0035707 |
| D. melanogaster: ed1X5 | Bai et al., 2001 | Flybase: FBal0086650 |
| D. melanogaster: btl-GAL4 | Bloomington Drosophila Stock Center | BDSC; 78328; Flybase: FBtp0001208 |
| D. melanogaster: UAS-CnoGFP | Perez-Gomez et al., 2013 | Flybase: FBal0294979 |
| D. melanogaster: cnoR2 | Bloomington Drosophila Stock Center | BDSC; 51328; Flybase: FBal0243335 |
| D. melanogaster: Df(pyd)B12 | Bloomington Drosophila Stock Center | BDSC; 51329; Flybase: FBal0285508 |
| D. melanogaster: UAS-pydRNAiHM05131 | Bloomington Drosophila Stock Center | BDSC; 28920; Flybase: FBtp0051218 |
| D. melanogaster: Ecad-GFP | Huang et al., 2009 | Flybase: FBtp0247909 |
| D. melanogaster: α-Cat::GFP | Bloomington Drosophila Stock Center | BDSC; 59405; Flybase; FBti0168861 |
| D. melanogaster: apmd544-GAL4 | Bloomington Drosophila Stock Center | BDSC; 3041; Flybase: FBal0051787 |
| D. melanogaster: UAS-Lifeact::GFP; sqh-Sqh::mCherry, GMR-GAL4 | Del Signore et al., 2018 | N/A |
| D. melanogaster: sqh-UtrABD::Cherry | Vazquez et al., 2014 | Flybase: FBal0298059 |
| Oligonucleotides | ||
| Sdk XbaI 5’ primer AGACTCTAGAGGAATCTATGGCCATG |
This paper | N/A |
| Sdk NheI 3’ primer CAGCTAGCGGCAGTGGTGCCCTC |
This paper | N/A |
| Sdk EcoRI 5’ primer ATCGGAATTCAAGAGCAAGAGCTATAAA |
This paper | N/A |
| Sdk NotI 3’ primer ATCGGCGGCCGCTCAGACGAATGACGAGAAGC |
This paper | N/A |
| SdkΔBD NotI 3’ primer ATCGGCGGCCGCTCAGAAGCCGG |
This paper | N/A |
| Pyd Myc EcoRI 5’ primer ACTGGAATTCCAAACATGGAGCAGAAGCTGATCTCCGAGGAGGACCTGGCTAGCGAGTACCACACGGTGGCGGTGA | This paper | N/A |
| Pyd XhoI 3’ primer ATCGCTCGAGTCAGCAGTACTGCACAATCAGATCGATGC |
This paper | N/A |
| Recombinant DNA | ||
| pUAS-Pyd-FLAGHA | Drosophila Genomics Resource Center | Cat # UFO07742 |
| pUAS-Myc-PydPDZ | This paper | N/A |
| pGEX-4T-Sdk | This paper | N/A |
| pGEX-4T-SdkΔBD | This paper | N/A |
| Software and Algorithms | ||
| ImageJ 1.50d | National Institutes of Health; Schindelin et al., 2012 | http://imagej.nih.gov.ij |
| GraphPad Prism 7.0c | GraphPad Software, Inc. | https://www.graphpad.com |
| Igor Pro 6.04 | WaveMetrics, Inc. | http://wavemetrics.com |