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. 2019 Sep 17;8:e49266. doi: 10.7554/eLife.49266

Figure 4. Kinetics of the initial working stroke displacement.

(a) and (b) Ensembles averages from events longer than 25 ms plotted after normalizing the amplitudes of the initial displacements. (a) without added Pi and (b) with 10 mM Pi for 1.5 pN to 3.75 pN hindering loads. (c) Observed stroke rates from single exponential fits for no added Pi (blue), and 10 mM added Pi (red). Error bars show 95% confidence intervals from the fits.

Figure 4—source data 1. Matlab figure files with data from Figure 4.
elife-49266-fig4-data1.zip (19MB, zip)
DOI: 10.7554/eLife.49266.014

Figure 4.

Figure 4—figure supplement 1. Models for the effect of free Pi on single-molecule displacements in the presence of hindering mechanical load.

Figure 4—figure supplement 1.

(a) If the working stroke occurs before Pi release, Pi is expected to cause more reversals and reversals which occur later in the events and would not delay the initial stroke. (b) If Pi is released before the working stroke, solution Pi is expected to delay the working stroke due to Pi binding to AM·ADP and then having to dissociate again before the stroke.