Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Pristionchus pacificus) | Ppa-daf-6p::rfp | this study | tuEx231 | extrachromosomal array transgenic strain |
| Genetic reagent (Pristionchus pacificus) | Ppa-daf-6p::venus | this study | tuEx250 | extrachromosomal array transgenic strain |
| Genetic reagent (Pristionchus pacificus) | Ppa-odr-3p::rfp | this study | tuEx265 | extrachromosomal array transgenic strain |
| Genetic reagent (Pristionchus pacificus) | Ppa-odr-7p::rfp | this study | tuEx296 and tuEx297 | extrachromosomal array transgenic strain |
| Genetic reagent (Pristionchus pacificus) | Ppa-che-1p::che-1:rfp | this study | lucEx367 | extrachromosomal array transgenic strain |
| Recombinant DNA reagent | Ppa-che-1 mRNA probe | Stellaris, Biosearch Technologies; this study | PPA01143 | single-molecule in situ fluorescence probe |
| Sequence-based reagent | Ppa-daf-6 promoter forward primer | this study | PPA15978 | CTCGCCCGTGGATCATGTG |
| Sequence-based reagent | Ppa-daf-6 promoter reverse primer | this study | PPA15978 | TGCAAATCATTGATTGAATCATGG |
| Sequence-based reagent | Ppa-odr-3 promoter forward primer | this study | PPA14189 | GAGCGAGTGAAATGAGCTCAGTCC |
| Sequence-based reagent | Ppa-odr-3 promoter reverse primer | this study | PPA14189 | GGGTGATCGATACGAGGAGTGTTC |
| Sequence-based reagent | Ppa-odr-7 promoter forward primer | this study | Contig1-aug1055.t1 | AACCAATGCATTGGCTTAGTTGGTTTCACTAATCACTACTG |
| Sequence-based reagent | Ppa-odr-7 promoter reverse primer | this study | Contig1-aug1055.t1 | CCCTTGTCATTCAGATGAGCGAGCTGATCAAGGAG |
| Sequence-based reagent | Ppa-che-1 promoter reverse primer | this study | PPA01143 | CAGGAAACAGCTATGACCATG |
| Sequence-based reagent | Ppa-che-1 intron reverse primer | this study | PPA01143 | CTGTGATAAGATCATTATTGGTAC |
| Chemical compound, drug | DiI | Molecular Probe | V22889 | 1:150 dilution |
| Chemical compound, drug | DiO | Molecular Probe | V22886 | 1:150 dilution |
| Software, algorithm | TrakEM2 | PMID: 22723842 | EM section alignment | |
| Software, algorithm | 3D reconstruction | bioRxiv 485771 | volumetric reconstruction | |
| Software, algorithm | Photoshop | Adobe | image processing | |
| Software | Image J | Imagej.net | image processing | |
| Algorithm | www.phylogeny.fr | PMID: 18424797 | maximum liklihood phylogeny |