De Munck 2019.

Methods	Study design: prospective Study grouping: parallel group
Participants	Baseline characteristics Number of participants: 99 Number of cycles: 99 Women aged between 24‐40 years old Inclusion criteria Participants who underwent ICSI with ejaculates (fresh or frozen) Cycles with ≥ 6 mature oocytes available for intracytoplasmic sperm injection Extended culture to day 5, either with fresh embryo transfer or with a freeze all strategy on day 5 Exclusion criteria: no other criteria
Interventions	Intervention characteristics 36.6°C 37.1°C (control group)
Outcomes	Primary outcome: fertilisation and embryo development (top and good quality) and utilisation rate (number of embryos transferred and cryopreserved per number of mature oocytes) up to day 5 or 6 Secondary outcome: Clinical pregnancy rate (presence of at least one gestational sac at ultrasonographic visualisation; multiple gestational sacs were counted as one clinical pregnancy) Pregnancy: positive βhCG blood test after 14 days after transfer Clinical pregnancy with foetal heart beat: presence of at least one viable foetus 5‐7 weeks after fresh embryo transfer
Notes	Quote: :The authors report no financial or commercial conflicts of interest."
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	Low risk	Quote: "Mature sibling oocytes were randomly allocated to one of the two study groups. The first half of the sibling oocytes was allocated to one specific group based on a computer‐generated randomisation list, whereas the second half of the sibling oocytes was allocated to the other group."
Allocation concealment (selection bias)	Unclear risk	Judgement comment: oocytes were allocated to the two groups based on a randomisation list. No precise information is described
Blinding of participants and personnel (performance bias) All outcomes	High risk	Judgement comment: participants from De Munck 2019 were blinded, but personnel were not blinded
Blinding of outcome assessment (detection bias) All outcomes	Low risk	Judgement comment: objective outcome
Incomplete outcome data (attrition bias) All outcomes	Low risk	Judgement comment: results described adequately
Selective reporting (reporting bias)	Unclear risk	Judgement comment: no protocol has been published
Other bias	High risk	Judgement comment: randomisation performed on oocytes, not on women. No miscarriage rates or live birth rates were reported.