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. Author manuscript; available in PMC: 2019 Sep 17.
Published in final edited form as: J Vis Exp. 2019 Jun 13;(148):10.3791/59815. doi: 10.3791/59815

Figure 4. Dynamic ASL pH measurement in response to CFTR activation by forskolin.

Figure 4

(A) Representative traces of the effect of forskolin (Fsk, 10 µM) on the kinetics of ASL pH over time in non-CF and CF hAECs. Data represent the mean ± SEM from n=3 experiments. (B) Summary of the effect of Fsk on ASL pH in non-CF and CF cultures. Data represent the mean ± SD from n=69 non-CF cultures and 35 CF cultures (2-way ANOVA, Sidak's multiple comparisons test). (C) Representative traces of the effect of CFTRinh172 (172, 20 µM) on the Fsk-induced increase in ASL pH in non-CF hAECs. Data represent the mean ± SEM from n=5 experiments. (D) Summary of the effect of 172 on Fsk-induced alkalinisation of the ASL in non-CF cultures. Data represent the mean ± SEM from n=5 experiments (2-way ANOVA, Sidak's multiple comparisons test).