KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Ebola GP [Clone: KZ52] | Absolute Antibodies | Cat# Ab00690–23.0 |
| Mouse anti-mouse neurofilament, heavy [Clone: SMI-31] | BioLegend | Cat# 801601; RRID:AB_2564641 |
| Mouse anti-mouse neurofilament, heavy [Clone: SMI-32] | BioLegend | Cat# 801701; RRID:AB_2564642 |
| Mouse anti-GAD67 [Clone: 1G10.2] | Millipore Sigma | Cat# MAB5406; RRID:AB_2278725 |
| Chicken anti-GFAP polyclonal antibody | Novus Biologicals | Cat# NBP1–05198; RRID:AB_1556315 |
| Mouse anti-NeuN [Clone: A60] | Millipore Sigma | Cat# MAB377; RRID:AB_2298772 |
| Goat anti-rabbit IgG Secondary Ab Alexa Fluor 568 | ThermoFisher | Cat# A11036; RRID:AB_10563566 |
| Goat anti-rabbit IgG Secondary Ab Alexa Fluor Plus 647 | ThermoFisher | Cat# A32733; RRID:AB_2633282 |
| Goat anti-chicken IgY Secondary Ab Alexa Fluor 488 | ThermoFisher | Cat# A11039; RRID:AB_2534096 |
| Goat anti-chicken IgY Secondary Ab Alexa Fluor 568 | ThermoFisher | Cat# A11041; RRID:AB_2534098 |
| Goat anti-chicken IgY Secondary Ab Alexa Fluor 647 | ThermoFisher | Cat# A21449; RRID:AB_2535866 |
| Goat anti-mouse IgG Secondary Ab Alexa Fluor 488 | ThermoFisher | Cat# A11017; RRID:AB_2534084 |
| Goat anti-mouse IgG Secondary Ab Alexa Fluor 568 | ThermoFisher | Cat# A11019; RRID:AB_143162 |
| Mouse anti-mouse neurofilament-160 [Clone: NN18] | Sigma | Cat# N5264; RRID:AB_477278 |
| Rat anti-mouse GFAP-eFluor 660 [Clone: GA5] | eBioscience | Cat# 50-9892-82; RRID:AB_2574408 |
| Goat anti-mouse alexa fluor 488 | Life Technologies | Cat# A11001; RRID:AB_2534069 |
| Goat anti-rabbit alexa fluor 555 | Life Technologies | Cat# A21428; RRID:AB_2535849 |
| Bacterial and Virus Strains | ||
| VSV (Indiana strain) | ATCC | VR-1238 |
| rVSVΔG-ZEBOV-GP | BEI Resources | N/A |
| rVSVΔG-ZEBOV-GPΔMUC | Gift: Gerardo Kaplan (this paper) | N/A |
| rVSVΔG-RESTON-GP | Gift: Keith Peden (Lee et al., 2017) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ProLong Diamond Antifade w/ DAPI | ThermoFisher | Cat# P36962 |
| Fluoro-jade C Stain | EMD Millipore | Cat# AG32530MG |
| DAPI | ThermoFisher | Cat# D3571 |
| DAPI | Thermo Scientific | Cat# 62248 |
| DPX Mountant for histology | Sigma | Cat# 06522 |
| Critical Commercial Assays | ||
| TaqMan Fast Advanced Master Mix | ThermoFisher | Cat# 4444557 |
| TURBO DNA-free Kit | ThermoFisher | Cat# AM1907 |
| High-Capacity cDNA Reverse Transcription Kit | ThermoFisher | Cat# 4368813 |
| ApopTag FITC in situ apoptosis detection kit | EMD Millipore | Cat# S7110 |
| Experimental Models: Cell Lines | ||
| VERO-E6 | ATCC | CRL-1586; RRID:CVCL_0574 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Cat# 000664; RRID:IMSR_JAX:000664 |
| Mouse: IFNAR1−/− (B6.129S2-lfnar1tm1Agt/Mmjax) | The Jackson Laboratory | Cat# 032045-JAX; RRID:MMRRC_ 032045-JAX |
| Mouse: RAG1−/− (B6.129S7-Rag1tm1Mom/J) | The Jackson Laboratory | Cat# 002216; RRID:IMSR_JAX:002216 |
| Mouse: MDA5−/− (B6.Cg-lfih1tm1.1Cln/J) | The Jackson Laboratory | Cat# 015812; RRID:IMSR_JAX:015812 |
| Mouse: IFNb−/− | Gift: Dr. Stefanie Vogel (Shirey et al., 2010) | N/A |
| Oligonucleotides | ||
| Cxcl10 mouse taqman primers | ThermoFisher | Cat# Mm00445235_m1 |
| Gapdh mouse taqman primers | ThermoFisher | Cat#Mm99999915_g1 |
| Ccl5 mouse taqman primers | ThermoFisher | Cat#Mm01302427_m1 |
| Irf7 mouse taqman primers | ThermoFisher | Cat#Mm00516793_g1 |
| Isg15 mouse taqman primers | ThermoFisher | Cat# Mm01705338_s1 |
| Software and Algorithms | ||
| Graphpad Prism v7.x | GraphPad software | N/A |
| Adobe Photoshop CC v20.x | Adobe | N/A |
| Adobe Illustrator CC v23.x | Adobe | N/A |
| Zeiss Zen v2 | Zeiss | N/A |
| Olympus VS Software v2.9 | Olympus | N/A |
| Quant-Studio Real-Time PCR System v1.2 | Thermo Fisher Scientific | N/A |
| ImageJ with Fiji v1.50d | NIH | https://imagej.nih.gov/ij/ |