Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 17;9:13486. doi: 10.1038/s41598-019-50004-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Concentration-dependent effects of HU 210 (A,E), HU 211 (B), CBD (C,E), and AEA (D) on the development and survival of chick embryos. The protective effects of 100 µM α-tocopherol (α-Toc.) or 1 µM AM251 against the embryotoxic effects produced by 10 µM HU 210 or 50 µM CBD are shown in (E). The embryos were treated in ovo with the test compounds by repeated injections (after 1, 4 and 7 days) in the yolk. On day 10, the eggs were cracked and embryos were analyzed in terms of their viability (as indicated in panels as percentage live embryos) and anatomical stage according to the Hamburger-Hamilton scale. Shown are dot plots with means ± SEM of 6–25 individual embryos per treatment. Statistically significant differences (one-way ANOVA with post-hoc Dunnett´s multiple comparison test) are indicated as: *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001, when the HH stage at each CB treatment group is compared with corresponding vehicle (0.05% DMSO or EtOH) controls, and in (E) when the combination of CB + α-Toc. or AM251 are compared with CB treatment per se.