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. 2019 Sep 17;9:13445. doi: 10.1038/s41598-019-49937-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of granulopoiesis only in bone marrow with EBV infection. (A) hCD45⁺ and hCD66b⁺ Abs were used for detection of granulocyte by flow cytometer. Bone marrow (BM) (EBV⁺; n = 16, EBV⁻; n = 6), SPL (spleen) (EBV⁺; n = 12, EBV⁻; n = 6) and PB (peripheral blood) (EBV⁺; n = 5, EBV⁻; n = 2) in mice were analyzed. (B) Frequency of granulocyte in humanized mice tissue. BM, SPL, PB were compared between infected and uninfected condition. Data are mean ± SD. (C,D) hCD33^intermediate cells n BM between EBV-infected (n = 10) and uninfected (n = 5) humanized mice BM. hCD33^intermediate cells were detected from hCD45⁺ cell population. **p < 0.05. (E) The majority of CD33^intermediate cells are hCD66⁺.