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. 2019 Sep 17;9:13471. doi: 10.1038/s41598-019-50029-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of peripheral T-cell subsets in FL patients before treatment. Box-and-whisker plots of flow cytometry data obtained from healthy donors (HD) and FL patients before treatment (FL-T0) blood samples. (a) Percentages of CCR7⁺CD45RA⁺ naive (T_N), CCR7⁻CD45RA⁻ (T_EM), CCR7⁺CD45RA⁻ (T_CM) and CD127⁻CD25⁺ (T_reg) CD4⁺ T cells. (b) Percentages of T_N, T_EM, T_CM and CCR7⁻CD45RA⁺ (T_EMRA) CD8⁺ T cells. (c) Percentages of CD38⁺HLA-DR⁺, PD-1⁺ and TIGIT⁺ among CD4⁺ and CD8⁺ T cells. (d) Percentages of PD-1⁺CTLA-4⁻, PD-1⁺CTLA-4⁺, CD45RA⁻ and CD26⁻CD39⁺ among T_reg. The number of samples that have been successfully processed are indicated below each panel. A Mann-Whitney test was performed for statistical analyses. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: not significant.