Fig. 2.
Neural JNK3 plays an important role during blood flow recovery after hindlimb ischemia (HLI). Time course of blood flow recovery by laser speckle contrast imaging of neural-specific nestin cre (a) and muscle-specific HSA cre (b) Mapk10−/− mice (Nes+/Cre, n = 9; Nes+/Cre Mapk10f/f, n = 8; HSA+/Cre, n = 8; HSA+/Cre Mapk10f/f, n = 8). c Representative figures of blood flow measurements in the HLI model model on day 7 post HLI in Nes+/Cre and Nes+/Cre; Mapk10f/f mice. d Expression of von Willebrand factor (vWF) in mouse thigh adductor muscle on day 21 after femoral artery ligation. e Quantification of the diameter of collateral vessels, vWF, in mouse thigh adductor (TA) muscle on day 21 after femoral artery ligation (Nes+/Cre control, n = 17; Nes+/Cre Mapk10f/f, n = 16; Nes+/Cre ischemia, n = 17; Nes+/Cre Mapk10f/f ischemia, n = 15). f qRT-PCR was performed for different genes related to collateral artery remodeling on thigh adductor muscle from the control and ischemic legs of WT and Nes+/Cre; Mapk10f/f mice 3 days post femoral artery ligation (n = 4 in each group). All the experiments have been repeated 3–6 times. Statistically significant differences between groups are indicated (*P < 0.05 by Student’s t test). The data are mean ± SEM. Scale bar, 50 μm. Source data are provided as a Source Data file