Fig. 3.
Suppression of JNK3 activates pro-angiogenic genes in gastrocnemius muscle during ischemia. a Microarray was performed on pooled (three mice) gastrocnemius muscle three days post femoral artery ligation. b–d qRT-PCR was performed for different blood flow recovery related genes on gastrocnemius muscle from control and ischemic legs of WT and Mapk10−/− mice 3 days post femoral artery ligation (n = 4 in each group). e qRT-PCR was performed for growth factor related genes on gastrocnemius muscle from control and ischemic legs of Nes+/Cre and Nes+/Cre; Mapk10f/f mice 3 days post femoral artery ligation (n = 4 in each group). f–h Gastrocnemius muscle was isolated and immunoblots were performed as described. All the experiments have been repeated 3–6 times. Statistically significant differences between groups are indicated (*P < 0.05 by Student’s t test). The data are mean ± SEM. Source data are provided as a Source Data file