Fig. 4.

JNK3 regulates pro-angiogenic factors expression in femoral nerve and Neuro-2a (N2a) cell lines. a Femoral nerves were isolated on day 3 after femoral artery ligation and qRT-PCR was performed for different blood flow recovery related genes (n = 3 in each group). b Following 90 min of hypoxia, RNA was isolated from control and JNK3 knockdown N2a cells and qRT-PCR was performed for genes as indicated (n = 4 in each group). c Femoral nerves were isolated from control and ischemic legs of mice after 48 h of gene painting with adenovirus expressing Mapk10 shRNA and qRT-PCR was performed for genes as indicated (n = 4 in each group). d Gastrocnemius muscle was isolated from control and ischemic legs of mice on day 3 after femoral artery ligation and qRT-PCR was performed as indicated. Statistically significant differences between groups are indicated (*P < 0.05 by Student’s t test). The data are mean ± SEM. Source data are provided as a Source Data file