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. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of split fluorescent proteins. a Single-molecule brightness measurement of split FPs and their full-length counterparts using fluorescence fluctuation spectroscopy. N = 10 (mNG2 and sfCherry5) or 15 (GFP) measurements. Error bars are standard deviations. See Supplementary Data for the list of data values. b–d Flow cytometry analysis of whole-cell fluorescence in HEK 293T expressing either b GFP_1–10/11, c mNG2_1–10/11, and d sfCherry2_1–10/11 or their full-length counterparts. The x-axis is the log-scale infrared fluorescence intensity indicating the expression level, and the y-axis is the log-scale green (or red) fluorescence intensity. The gray dashed trend lines have a slope of 1 and intercepts are set to best follow the points in the right (full-length) panels. The pink dashed trend lines have a slope of 2 and intercepts set to best follow the points in the left (split) panels with the exception of GFP_1–10/11. e Expression levels of two fragments are proportional within a wide range of expression levels in a co-transfect experiment. The gray dashed trend line has a slope of 1