Fig. 2.

SpyTag/SpyCatcher-assisted complementation of split sfCherry2_1–10/11. a Schematic diagram of SpyTag/SpyCatcher-assisted complementation and construct design. b Flow cytometry analysis of whole-cell fluorescence in HEK 293T cells expressing SpyTag-sfCherry2₁₁-TagBFP with either sfCherry2_1–10 alone or with SpyCatcher-6aa-sfCherry2_1–10. The gray dashed trend line has a slope of 1 and the pink one has a slope of 2. c Fluorescence microscopy of sfCherry2₁₁ labeled H2B without or with the assistance of SpyTag/SpyCatcher interaction. The imaging condition and brightness/contrast range were set the same for better comparison. Scale bars: 50 µm