Fig. 5.

NLG-1 CLASP visualizes specific subsets of synapses in live C. elegans. a, b Schematic diagrams of GFP_1–10/11-based NLG-1 GRASP and sfCherry3_1–10/11-based NLG-1 CLASP expressed in PHB and AVA neurites. c, d Schematics and micrographs of NLG-1 GRASP and NLG-1 CLASP specifically labeling synaptic contacts between the two PHB and the two AVA neurons in C. elegans (one neuron from each pair is shown for simplicity). PHB-AVA NLG-1 GRASP and NLG-1 CLASP fluorescence intensity are dramatically reduced in the synaptic partner recognition mutant clr-1/RPTP. e, f Quantification of the reduction in relative fluorescence intensities of NLG-1 GRASP and NLG-1 CLASP in clr-1/RPTP mutants. N = animals in each case, ***P < 0.001, **P < 0.01, Mann–Whitney U-test. See Supplementary Data for the list of data values