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. 2019 Sep 11;10:2144. doi: 10.3389/fimmu.2019.02144

Figure 3.

Figure 3

Neutrophil progenitors isolated by density centrifugation show a less effective MDSC activity. (A) Mature neutrophils (black bars) and neutrophil progenitors from the bone marrow pellet fraction after density centrifugation (gray bars) were stimulated with the indicated stimuli and production of H2O2 was determined by measuring Amplex Red conversion into fluorescent Resorufin (n = 3). (B) Purified CFSE-labeled T cells from healthy donors were cultured with anti-CD3 and anti-CD28 antibodies (white bars, n = 6), and in presence of mature neutrophils from blood (black bars, n = 6) or neutrophil progenitors from the bone marrow pellet (gray bars, n = 3) and/or indicated stimuli. Cells were harvested after 5–6 days and analyzed by flow cytometry for CFSE dilution among CD4+ T cells. Error bars indicate SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.