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. 2019 Sep 11;10:2144. doi: 10.3389/fimmu.2019.02144

Figure 4.

Figure 4

Only the CD16+ neutrophil progenitors show MDSC activity, ROS production and degranulation. (A,B) CD16 positive cells were isolated via MACS isolation from mature neutrophils from blood and neutrophil progenitors from BM pellet. (A) Purified CFSE-labeled T cells from healthy donors (n = 4) were cultured with anti-CD3 and anti-CD28 antibodies (white bars), and in presence of mature neutrophils from control donors (black bars, n = 6), CD16+ mature neutrophils (gray bars, n = 6), total BM pellet fraction (dark green bars, n = 4), CD16+ (green bars, n = 4) or CD16− (light green bars, n = 4) progenitors from BM pellet and/or indicated stimuli. Cells were harvested after 5–6 days and analyzed by flow cytometry for CFSE dilution among CD4+ T cells. (B) The indicated cell fractions were stimulated with the indicated stimuli and production of H2O2 was determined by measuring Amplex Red conversion into fluorescent Resorufin (n = 4–6). Error bars indicate SEM; *p < 0.05, **p < 0.01, ****p < 0.0001.