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. 2019 Sep 11;9:862. doi: 10.3389/fonc.2019.00862

Figure 2.

Figure 2

OTUD7B promotes proliferation, migration and colony formation in lung cancer cells. (A,B) NCI-H358 lung cancer cells were transduced with GFP or GFP-OTUD7B (A) or transduced with a non-targeting control shRNA (shNC) or two different OTUD7B-specific shRNAs (B). Cells proliferation was tested using MTT assay and the absorbance was measured at 490 nm. (C) NCI-H358 cells were transduced with GFP or GFP-OTUD7B. Cell proliferation were determined by colony formation analysis. (D,E) Wound healing assay (D) and transwell invasion assay using Boyden chamber (E) in NCI-H358 cells were performed and photographed under a light microscope (magnification, ×100). Representative images are shown (upper panels). Data are shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.