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. 2019 Sep 17;9:13495. doi: 10.1038/s41598-019-49756-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cMet agonistic antibody on kidney fibrosis and wound healing in primary cultured human proximal tubular epithelial cells (PTECs) in vitro. (A) Representative confocal images of primary cultured human PTECs costained for DAPI (blue), fibronectin (green), and collagen IV (red). Fibrosis was induced with TGFβ, and treatment with the cMet agonistic Ab (500 ng/ml) indicated improvements in fibrosis. Original magnification: X200. (B,C) In the presence or absence of either rHGF (10, 20 ng/ml) or cMet Ab (250, 500, 1000 ng/ml) for 30 minutes, rTGFβ-stimulated PTECs exhibited significantly increased protein levels of fibronectin, αSMA and Bax2. (D,E) The migratory capacity of PTECs was assessed using a scratch wound healing assay and observing cell movement at 0 and 10 hours after scratching. There was a significant difference in the migratory potential of cells in the high-dose cMet Ab group compared to that in the control groups. Data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 versus control group.