Table 2.
Antibodies used in immunohistochemistry, immunofluorescence staining and immunoblotting.
| Figure in the text | Antibody (vendors) | Catalog number |
|---|---|---|
| 1A | anti-Met (cMet) Ab (Abcam) | ab59884-100 |
| 1B | DAPI (Molecular Probes) | D1306 |
| anti-Met (cMet) Ab (Abcam) | ab59884-100 | |
| anti-aquaporin-1 Ab (Abcam) | ab15080 | |
| Alexa Fluor-conjugated secondary Ab (Molecular Probes). | ||
| goat anti-mouse 488 (green) | A11001 | |
| goat anti-mouse 555 (red) | A21422 | |
| goat anti-rabbit 488 | A11008 | |
| goat anti-rabbit 555 | A21428 | |
| anti-Met (cMet) (phospho Y1349) Ab (Abcam). | ab68141 | |
| 1C | rHGF (R&D Systems) | 294-HG-005 |
| 2A–E | cMet Ab (Abcam) | ab59884-100 |
| Anti-Hunan IgG Ab (Abcam) | ab200699 | |
| Anti-Human HGF R/cMet PE-conjugated Ab | FAB3582P | |
| 3A–H | Anti-Hunan IgG Ab (Abcam) | ab200699 |
| cMet Ab (Abcam) | ab59884-100 | |
| DAPI (Molecular Probes) | D1306 | |
| 4B | anti-Met (cMet) Ab (Abcam) | ab59884-100 |
| anti-aquaporin-1 Ab (Abcam) | ab15080 | |
| anti-rabbit (Cell Signaling Technology) secondary | 7074 s | |
| 4C | anti-mouse (Cell Signaling Technology) Secondary | 7076 s |
| anti-collagen 1 Ab (Abcam) | Ab90395 | |
| anti-fibronectin Ab (Santa Cruz Biotechnology) | sc-8422 | |
| anti-αSMA Ab (Abcam) | ab7817 | |
| anti-beta-actin Ab (Cell Signaling Technology) | #4967 | |
| anti-Met (cMet) (phospho Y1349) Ab (Abcam). | ab68141 | |
| 5A | anti-Met (cMet) Ab (Abcam) | ab59884-100 |
| anti-beta-actin Ab (Cell Signaling Technology) | #4967 | |
| DAPI (Molecular Probes) | D1306 | |
| 6A | anti-fibronectin Ab (Santa Cruz Biotechnology) | sc-8422 |
| anti-collagen 4 Ab (Abcam) | ab21295 | |
| anti-fibronectin Ab (Santa Cruz Biotechnology) | sc-8422 | |
| 6B | anti-αSMA Ab (Abcam) | ab7817 |
| anti Bax Ab (Santa Cruz Biotechnology) | sc-7480 | |
| anti-Bcl-2 Ab (Santa Cruz Biotechnology) | sc-7382 | |
| anti-Met (cMet) (phospho Y1349) Ab (Abcam). | ab68141 | |
| anti-Met (cMet) Ab (Abcam) | ab59884-100 | |
| anti-beta-actin Ab (Cell Signaling Technology) | #4967 | |
| 6D | DAPI (Molecular Probes) | D1306 |
HRP; horseradish peroxidase, IHC; immunohistochemistry, IF; immunofluorescence, DAPI; 4′,6-Diamidino-2-Phenylindole, Dihydrochloride, PTECs; proximal tubular epithelial cells, rTGFβ; recombinant transforming growth factor β, rHGF; recombinant hepatocyte growth factor, FACS; fluorescence-assisted cell sorting.