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. 2019 Sep 11;10:1002. doi: 10.3389/fphar.2019.01002

Figure 1.

Figure 1

Elevated IL-6 expression and inhibition of IL-6 expression by apigenin in human esophagus cancer Eca-109 and Kyse-30 cells. (A) The IL-6 expression level in normal and tumor tissues of esophagus cancer patient samples (left panel) and the relationship between patient survival and IL-6 expression by Kaplan-Meier estimator with post hoc log-rank test (right panel). (B) Western blotting analysis of Eca-109 (left panel) and Kyse-30 cells (right panel) treated with various concentrations of apigenin. (C) ELISA analysis of the culture medium collected from Eca-109 (left panel) and Kyse-30 cells (right panel) treated with various concentrations of apigenin. (D) The relative luciferase activities in Eca-109 (left panel) and Kyse-30 cells (right panel) transiently transfected with IL-6 promoter luciferase reporter plasmid and then treated with the indicated concentrations of apigenin. *P < 0.05 indicates significant difference as compared to the corresponding control (n = 3). *P < 0.05 and **P < 0.01 indicate significant difference as compared to the corresponding control (n =3).